Journal: Pharmaceutics
Article Title: Hyaluronic Acid-Based Nanoparticles Loaded with Rutin as Vasculo-Protective Tools against Anthracycline-Induced Endothelial Damages
doi: 10.3390/pharmaceutics16080985
Figure Lengend Snippet: ( a ) NLRP-3 and ( b ) MyD-88 expression (ng/mL) in human endothelial cells incubated with epirubicin, Free-Rutin at 1 and 10 µM, LicpHA or LicpHA Rutin at 1 and 10 µM alone or combined with epirubicin. The error bars depict means ± SD. p -values for the indicated compounds relative to untreated cells are *** p < 0.001. ** p < 0.01. * p < 0.05. ns, not significant.
Article Snippet: Lysates were centrifuged supernatants and were used for the quantification of NLRP-3 through an NLRP-3 ELISA Kit (code OKEH03368, Aviva Systems Biology, San Diego, CA, USA) and MyD-88 through a MyD-88 ELISA Kit (code ab171341, Abcam, Milan, Italy).
Techniques: Expressing, Incubation